Real-Time PCR: Analyte-Specific Reagents versus FDA-Approved Kits
نویسندگان
چکیده
منابع مشابه
Evaluation of real-time PCR laboratory-developed tests using analyte-specific reagents for cytomegalovirus quantification.
Viral load testing for cytomegalovirus (CMV) has become the standard for the diagnosis of infection and monitoring of therapy at many transplant centers. However, no viral load test has been approved by the FDA. Therefore, many laboratories rely on laboratory-developed assays. This study evaluated the performance characteristics of two real-time PCR tests developed using the artus CMV analyte-s...
متن کاملInfluence of reagents formulation on real-time PCR parameters.
Real-time polymerase chain reaction (PCR) techniques are increasingly used to quantify target sequences for diagnostic and research purposes. Due to its 'quantitative' character, it is very important to determine the variability of this technique correlating with several experimental conditions. The objective of this study was to analyse the effect of manufacturing lots of PCR reagents on two m...
متن کاملDevelopment of a BK virus real-time quantitative assay using the bioMérieux analyte-specific reagents in plasma specimens.
OBJECTIVES Viral load testing for BK virus (BKV) has become the standard of care for diagnosing BKV infection and monitoring therapy in kidney transplant patients. However, there are currently no US Food and Drug Administration-approved assays and no standardization among available tests. METHODS This study evaluated the performance of the analyte-specific reagent (ASR) BKV primers r-gene and...
متن کاملConventional multiplex polymerase chain reaction ( PCR ) versus real - time PCR for species - specific meat
Simple and reliable conventional multiplex-polymerase chain reaction (multiplex-PCR) as well as real-time PCR approaches for the partial-length cytochrome b or cyt b gene of mitochondrial DNA (mtDNA) were compared to identify seven animal species for the authenticity of low non Halal (pig, dog, cat and donkey) and high value Halal (cattle, sheep and goat) meats. Either analysis indicated the su...
متن کاملInfluence of PCR reagents on DNA polymerase extension rates measured on real-time PCR instruments.
BACKGROUND Radioactive DNA polymerase activity methods are cumbersome and do not provide initial extension rates. A simple extension rate assay would enable study of basic assumptions about PCR and define the limits of rapid PCR. METHODS A continuous assay that monitors DNA polymerase extension using noncovalent DNA dyes on common real-time PCR instruments was developed. Extension rates were ...
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ژورنال
عنوان ژورنال: Microbe Magazine
سال: 2006
ISSN: 1558-7452,1558-7460
DOI: 10.1128/microbe.1.209.3